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I. Basic PrincipleFrozen sections are rapid tissue sections obtained by cutting frozen tissue to the desired thickness (5-20μm) within a constant-temperature freezer (-15 to -23°C). The rapid freezing of tissue samples transforms water into ice, with the solid ice within the tissue acting ...
One,PrincipleFrozen sections are a method that rapidly cools tissue to a certain hardness under low temperatures, and then slices it. In simple terms, paraffin sections are used for immunohistochemical experiments, while frozen sections are commonly used for immunofluorescence experiments.In froz...
(Preparation before paraffin sectioning) 1. Prior to use, it is crucial to inspect the blade's damage under a microscope and then sharpen it. Now, disposable blades are also available, which can eliminate the need for sharpening. 2. Trim Paraffin Blocks: Remove excess paraffin surrounding the tis...
Paraffin embedding is the most commonly used method in routine pathological work. The melting point of the paraffin used for embedding is generally around 60℃. In some cases, such as during certain enzyme stains, low-temperature paraffin embedding is required. In such instances, paraffin w...
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