Anaerobic Glove Box YQX-I Oxygen Concentration Display

Anaerobic Glove Box YQX-I Oxygen Concentration Display

An anaerobic incubator is an experimental device used for bacterial cultivation and operations under anaerobic conditions. It provides a strictly anaerobic, constant-temperature cultivation environment and a systematically and scientifically designed work area. Operating cultures within an anaerobic incubator allows for the cultivation of difficult-to-grow anaerobic organisms, while also avoiding the risk of death from oxygen exposure that anaerobic organisms faced during atmospheric operations. Therefore, this device is an ideal tool for anaerobic biological detection research. It is also a versatile instrument, as it can be adapted to provide optimal growth conditions for microaerophilic bacteria by changing the operational method.

Key Features:

1. The anaerobic incubator is composed of the culture operation room, vacuum sampling room, gas circuit, electrical control system, and other components. The whole machine features a novel design, compact structure, and boasts excellent anaerobic conditions, good sealing performance, high temperature control accuracy, good stability, ease of use, energy-saving, cost-effective, and safe and reliable operation. Its characteristics include:

Achieve high precision in anaerobic environments using state-of-the-art scientific methods; maintains stability and is reliable in use.

2. The incubator temperature control utilizes a high-precision digital display regulator, accurately and intuitively reflecting the internal temperature of the box. It can automatically control the temperature, serving as an effective temperature-limiting protection device to ensure the growth of the culture in a safe temperature environment.

3. The box contains an ultraviolet sterilization lamp, which effectively prevents contamination by pathogens.

4. Gas line equipment, capable of precisely adjusting flow rates at will and accommodating any required gas input.

5. The airway switch utilizes a locking switch to control the solenoid valve, offering flexible operation.

6. The front window of the control room is made of thick transparent special glass, allowing for a clear and direct observation of the operations inside. Operators use plastic gloves for reliable, comfortable, and flexible handling, making usage convenient.

7. Equipped with an oxygen removal catalyst indoors, it can completely eliminate the residual oxygen in the culture operation room.

8. The incubator features an over-temperature alarm function. When the internal temperature exceeds the set alarm value, it automatically cuts off the heating circuit and emits a visual alarm signal.

Instructions for Proper Installation of an Anaerobic Incubator

Close and seal the doors of the sampling room, both inside and out, and perform a vacuum check.

2. Connect and inspect the air line, and seal the joints with sealant as necessary to prevent leaks; To form an anaerobic environment in the operation room: place the necessary accessories and equipment according to the usage requirements, and put two non-toxic plastic bags into the operation room; Turn on the power to switch on the lighting, turn on the temperature controller, and adjust the required temperature and safety temperature;

3. Turn on the UV sterilization lamp for indoor sterilization, with the sterilization time based on specific experiments.

4. The anaerobic incubator should be placed in a location with minimal temperature variation and easy to operate, avoiding direct sunlight and keeping a distance from heating equipment, and should be placed flatly; securely position the mixed gas cylinder and nitrogen cylinder flatly, and install the pressure regulators (including pressure gauges) separately, and place them in an appropriate location.

5. Operation Room Nitrogen Displacement: A. Insert a rubber hose into the operation room's intake port, and the other end into a plastic bag; B. Connect the nitrogen intake line, open the nitrogen control valve, fill both plastic bags with nitrogen, close valve 1, and then tie the bag shut; C. Place the latex gloves over the observation panel flange and secure them; D. Gradually release the nitrogen from the plastic bag into the operation room until all is dispersed.

6. Open the oxygen scavenger in the operating room, connect the power to the oxygen scavenging catalyst, and perform catalytic oxygen removal. After one hour, open the Methylene Blue indicator and observe its color change; no color change indicates that the operating room has reached an anaerobic environment.

7. Operation Room Third Gas Exchange (Mixed Gas Exchange): A. Open the gas circuit and turn on the mixed gas valve to allow air intake. During inflation, first evacuate the sampling chamber to a vacuum state and then use the foot switch to control exhaust as needed; B. After the mixed gas fills the plastic bag, close the mixed gas valve and open the valve to allow the mixed gas to pass through the flowmeter. Adjust the flowmeter to maintain a flow rate of approximately 10ml per minute; C. Gradually release the mixed gas inside the plastic bag into the operation room; D. After three gas exchanges, the oxygen content in the operation room is at an extremely low level.

8. The control room D performs a second nitrogen displacement (nitrogen replacement) by repeating the nitrogen filling process. The sampling room is first evacuated, and attention is given to using the foot switch to open and close the exhaust at all times.

An anaerobic incubator is a specialized device designed for culturing and operating bacteria in an oxygen-free environment. It can cultivate difficult-to-grow anaerobic organisms and also avoids the risk of anaerobic organisms dying when exposed to oxygen during operations in the atmosphere.
Anaerobic incubators, also known as anaerobic workstations or anaerobic glove boxes, are specialized devices used for bacterial culture and operations under anaerobic conditions. They provide a consistent temperature-controlled environment for strict anaerobic states and feature a systematic, scientific working area.
This type of product typically consists of an environmentally controlled culture room, anaerobic operation room, sampling room, gas and electrical control system, cabinet racks, bottle racks, wax melting sterilizers, and other components.
Due to the inconvenience in the structural design, the anaerobic workstation has undergone revolutionary changes to meet the many usage experience demands of the operators.
It is divided into four main parts: the anaerobic chamber, the transmission compartment, the bare sleeve operation port, and the air and electrical control system.
Anaerobic chamber
Internal cavity mechanical forced convection and positive pressure ensure stable and uniform conditions for constant temperature, humidity control, oxygen removal, and biological detoxification, with rapid recovery. Operation and culture are conducted in the same room.
2. Transmission compartment:
Compact cylinder design allows for easy single-handed transfer of samples by one person.
3. Raw sleeve cuff operating hole:
No vacuum/purge process required; hands can directly enter and exit the internal cavity anaerobically.
4. Control System:
Real-time status self-inspection and alarm function to ensure the equipment operates normally.

Anaerobic Glove Box YQX-I Oxygen Concentration Display

Technical Specifications: