How to Use a Microscope and Points to Note

(1) Microscope Usage and Proper Installation Issues

Before using the microscope, it is necessary to first install the eyepiece and objective lens.How to Use a MicroscopeThe installation of the eyepiece is quite simple; the main issue lies with the installation of the objective lens. Since the objective lens is valuable, it's easy to drop it if the threads aren't properly aligned during installation, potentially damaging the lens. For safety, when installing the target, use your left index finger and middle finger to support the objective lens, and then install it with your right hand. This way, even if it's not fully installed, it won't fall to the ground.

(II)How to Use a MicroscopeAddressing issues with light and angle accuracy

Light collection is a crucial step in the use of microscopes. It is essential to use a low-power lens for light collection. When the light is strong, use a small aperture and a flat mirror; when the light is weak, use a large aperture and a concave mirror. Turn the lens with both hands until a uniformly bright circular field is observed. After aligning the light, do not move the microscope randomly to prevent the light from failing to pass through the lens and enter the light aperture accurately.

(3) How to use a microscope and the issue of correctly adjusting the focal distance screw

The use of a microscope involves adjusting the focus with the fine adjustment screw to locate the image, which is an essential step in microscope operation. Common mistakes include: first, directly focusing at high magnification; second, regardless of whether the tube is rising or falling, the eyes are always looking through the eyepiece; third, we are unaware of the critical distance for the object, and the object distance is adjusted to 2 to 3 centimeters but still rising, with the speed of turning the fine adjustment screw being very fast.

　　How to Use a MicroscopeThe first two types of errors often lead to collisions between the objective lens and the loader, damaging either the loader or the lens. The third type of error is a common phenomenon during the use of microscopes. In light of these errors, it is crucial to be cautious when adjusting the focal length. When using the coarse adjustment, first lower the lens, then slowly turn the coarse screw on the condenser and lens slides. Be careful not to let the lens touch the slide. During this process, keep an eye on the objective side and then use your left eye to focus through the eyepiece, slowly reversing the coarse adjustment to raise the tube until the image is clear.

Objects under a standard microscope are typically around 1 centimeter in distance. Therefore, if the distance exceeds 1 centimeter and the object is still not visible, it may be due to the specimen not being in the field of view or the coarse focus screw being turned too quickly. At this point, you should adjust the position and repeat the above steps. As the view of the blurred object changes, adjust the focus screw accordingly. Only then can you narrow down the search area and speed up the process.

Section 4: Microscope Usage and Objective Lens Conversion Issues

To use a microscope, first use the low-power lens and then switch to the high-power lens. Do not use your fingers to push the objective lens, as this can easily cause the optical axis of the objective lens to misalign. The reason is that the converter material is soft, with high precision, and the threads can become loose due to uneven force distribution. If the threads break, the entire converter will be报废. To switch the objective lens, hold the rotating lever below the converter. Stay tuned to Bifidi for more information.How to Use a MicroscopeRelated information.