Four Components of the Tissue Culture Room: Buffer Room, Sterile Room, Culture Room, and Seedling Room

In fact, the tissue culture room, like other factory workshops, is composed of specific areas. These typically include buffer rooms, sterile rooms, culture rooms, and seedling rooms.

  Buffer Room:Change clothes and shoes before entering the lab to minimize the introduction of contaminants. Consider installing UV sterilization lamps in the buffer room for照射 and sterilization.

  2. Sterile Room:Primarily used for disinfection, inoculation, culture transfer, tube seedling subculture, protoplast preparation, and all procedures requiring sterile operations in plant materials. The main equipment is a clean bench.

 3. Growth Room:The location for the growth of vaccination materials. Its size can be determined based on the dimensions and quantity of auxiliary equipment such as incubation racks. The main instruments and equipment include incubation racks and light incubators.

  4. Seedling RoomThe forced acclimatization primarily for aseptic seedlings, enabling them to rapidly adapt to the harsh environmental conditions in the field after planting.

The entire lab is partitioned with plastic steel glass, requiring a roof addition and air conditioning. The walls need to be transformed into a coating layer, and the ceiling should be made of smooth, dust-free materials.

  Expanded Information

During the tissue culture process, the reuse of vessels, particularly the attachment of organic matter in culture media and secretions of the cultured tissues, affects re-cultivation, leading to erroneous experimental results and even toxicity in the cultured tissues. Additionally, some new glassware contains non-alkaline substances, which also impacts the accuracy of experimental outcomes.

Therefore, new or reusable tableware must be thoroughly cleaned to meet the requirement of leaving no residue. Since different culture vessels vary in materials, structures, and usage methods, the cleaning procedures also differ.

In summary,Tissue Culture RoomConstructionThere are typically four components, each with its specific role, to ensure the culture room remains uncontaminated, thereby protecting the integrity of experimental data.