The process and steps for the purification of the tissue culture room

The process and steps for the purification of the tissue culture room mainly include the following aspects:

1. Cleaning and Disinfection：

• Before each day's experiment, clean the lab surfaces and equipment with sterile detergents to remove any impurities and bacteria from the previous day.

• During the experiment, regular disinfection of equipment, utensils, and tools is conducted to prevent contamination by bacteria and viruses.

• After the experiment, immediately carry out a thorough cleaning and disinfection of all used equipment, vessels, and tools to ensure cleanliness and safety for the next experiment.

• Disinfect the tissue culture room by spraying with 70% alcohol or a 0.1% solution of new洁尔灭, or use an ultraviolet sterilization lamp for air disinfection.

2. Sterile Technique：

• Our company strictly adheres to aseptic operation standards, including laboratory entry and exit procedures, attire requirements for lab coats, masks, and gloves, as well as sterilization rules for laboratory equipment.

• Maintain the closure of the laboratory, minimize the movement of personnel and items in and out, and avoid introducing external contaminants.

• After the experiment, the lab was disinfected with air to eliminate potential sources of contamination.

3. Air Purification：

• The tissue culture room should be equipped with an air purifier or air conditioning system to ensure clean and purified air.

• Regularly inspect and clean air purifier equipment to ensure proper operation and purification effectiveness.

• Install air filters at the intake to minimize the entry of external dust and bacteria, and regularly replace the filters to ensure their filtering efficiency.

Furthermore, when constructing a tissue culture room, it is crucial to pay attention to the design and installation of an air purification system, including the setup and adjustment of air filters, air circulation systems, supply air outlets, and return air inlets, to ensure the smooth operation of the air purification system and meet purification requirements.

Please note that the specific steps for the purification of the tissue culture room may vary depending on the specific conditions of the laboratory. To ensure the effectiveness of the purification, it is recommended to follow the relevant regulations and operational procedures of the laboratory and make appropriate adjustments and improvements based on the actual situation.