Portant; > Circular Bichromatic SpectrumSpatial configurations of organic compounds and biomacromolecules are studied. Optical active substances have unequal absorption coefficients (ε) for the composition of plane-polarized light and right-handed circularly polarized light, εL ≠ εR, indicating circular dichroism. If a graph is plotted with the wavelength λ of plane-polarized light of different wavelengths as the abscissa and the difference in absorption coefficients Δε = εL - εR as the ordinate, the resulting graph is the circular dichroism spectrum, abbreviated as CD. If a chiral compound absorbs in the UV-visible region, it can produce a characteristic circular dichroism spectrum. Due to εL ≠ εR, the transmitted light is no longer plane-polarized but elliptically polarized, and the molar ellipticity [θ] is related to Δε as [θ] = 3300Δε. The circular dichroism spectrum can also be plotted with molar ellipticity as the ordinate and wavelength as the abscissa. Since Δε can be positive or negative, the circular dichroism spectrum can also be positive circular dichroism, which appears as peaks, and negative circular dichroism, which appears as valleys. The determination of circular dichroism and rotational spectroscopy in the UV-visible region can infer the configuration and conformation of organic compounds.

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