The method for detecting ethanol content in blood involves using tert-butanol as an internal standard, followed by analysis with a headspace gas chromatography flame ionization detector. The sample is compared to a parallel ethanol standard using the internal standard method to quantify the peak area ratio of ethanol to the internal standard. The target peak and internal standard peak are well-separated on the chromatogram, and the standard working curve shows a good linear relationship within the range of 0.01 to 10.0 g/L. The headspace gas chromatography method for measuring ethanol content in blood is rapid and sensitive, allowing for accurate quantification. This method is suitable for actual detection and analysis.

The level of ethanol concentration in the blood is a significant indicator of an individual's level of consciousness.