The method for detecting ethanol content in blood involves using tert-butanol as an internal standard, followed by analysis of the sample using a headspace gas chromatography flame ionization detector. The results are quantified by comparing the peak area ratio of ethanol to the internal standard using the internal standard method. The target peak and internal standard peak are well-separated on the chromatogram, and the standard working curve exhibits a good linear relationship within the range of 0.01 to 10.0 g/L. The headspace gas chromatography method for determining blood ethanol content is rapid, sensitive, and provides accurate quantification, suitable for actual detection and analysis.

The level of ethanol concentration in the blood is a crucial indicator for determining an individual's level of sobriety.